SCREENING FOR MILK-CLOTTING ENZYME FROM BASIDIOMYCETES

被引：5

作者：

KOBAYASHI, H ^{[1
]}

KIM, H ^{[1
]}

ITOH, T ^{[1
]}

KASAMO, K ^{[1
]}

KUSAKABE, I ^{[1
]}

MURAKAMI, K ^{[1
]}

机构：

[1] UNIV TSUKUBA, INST APPL BIOCHEM, IBARAKI, OSAKA 305, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 02期

关键词：

D O I：

10.1271/bbb.58.440

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Screening tests for aspartic proteinases with milk-clotting activity were done on basidiomycetes. Crude enzymes from 6 strains had a high ratio of milk-clotting activity to caseinolytic activity. These enzymes showed acidic pH optimum for proteolytic activity and were inhibited considerably by pepstatin, a specific aspartic proteinase inhibitor. Among them, the crude enzyme from Laetiporus sulphureus was more heat-lahile than the other enzymes.

引用

页码：440 / 441

页数：2

共 5 条

[1]

Arima K., 1970, METHODS ENZYMOLOGY, V19, P446, DOI 10.1016/0076-6879(70)19033-1

[2] STUDIES ON MILK CLOTTING ENZYMES PRODUCED BY BASIDIOMYCETES .1. SCREENING TESTS OF BASIDIOMYCETES FOR PRODUCTION OF MILK CLOTTING ENZYMES [J].

KAWAI, M ;

MUKAI, N .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1970, 34 (02) :159-&

[3]

KIKUCHI E, 1988, AGR BIOL CHEM TOKYO, V52, P1277

[4] CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR IRPEX-LACTEUS ASPARTIC PROTEINASE [J].

KOBAYASHI, H ;

SEKIBATA, S ;

SHIBUYA, H ;

YOSHIDA, S ;

KUSAKABE, I ;

MURAKAMI, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (07) :1927-1933

[5] PURIFICATION AND CHARACTERIZATION OF 2 MILK-CLOTTING ENZYMES FROM IRPEX-LACTEUS [J].

KOBAYASHI, H ;

KUSAKABE, I ;

MURAKAMI, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (03) :551-558

← 1 →