Scanning electron microscopy of synaptonemal complexes

被引：7
|
作者：
Barlow, A. L. [1 ]
Jenkins, G. [2 ]
ap Gwynn, I. [3 ]
机构：
[1] Univ Glasgow, EM Ctr, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Wales, Dept Agr Sci, Aberystwyth SY23 3DD, Dyfed, Wales
[3] Univ Wales, Dept Biol Sci, Aberystwyth SY23 3DD, Dyfed, Wales
来源：
CHROMOSOME RESEARCH | 1993年 / 1卷 / 01期
关键词：
rat; rye; scanning electron microscopy; synaptonemal complex;
D O I：
10.1007/BF00710602
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The novel application of scanning electron microscopy to study whole-mount surface-spread synaptonemal complex complements of rye (Secale cereale) and rat (Rattus norvegicus) is described. Scanning electron microscopy is able to resolve the third dimension in such preparations and improve the tracing of the continuity of lateral elements without losing information that could be obtained by conventional transmission electron microscopy. This improvement is likely to benefit detailed studies of chromosome synapsis and karyology, and may provide a means of circumventing technical obstacles inhibiting the use of surface-spreads as substrates for in situ hybridization under the electron microscope.
引用
收藏
页码：9 / 13
页数：5
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