FLOW-INJECTION ANALYSIS WITH ELECTROCHEMICAL DETECTION FOR DETERMINATION OF SALICYLIC-ACID IN PHARMACEUTICAL PREPARATIONS

被引：26

作者：

NEUMAYR, M

FRIEDRICH, O

SONTAG, G

PITTNER, F

机构：

[1] UNIV VIENNA,INST ANALYT CHEM,WAHRINGERSTR 38,A-1090 VIENNA,AUSTRIA

[2] UNIV VIENNA,INST GEN BIOCHEM,A-1090 VIENNA,AUSTRIA

[3] UNIV VIENNA,LUDWIG BOLTZMANN FORSCHUNGSSTELLE,A-1090 VIENNA,AUSTRIA

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 273卷 / 1-2期

关键词：

AMPEROMETRY; ENZYMATIC METHODS; FLOW INJECTION; PHARMACEUTICALS; SALICYLIC ACID;

D O I：

10.1016/0003-2670(93)80192-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme-based flow-injection system with electrochemical detection suitable for the determination of salicylic acid in pharmaceuticals is described. The method makes use of the enzyme salicylate hydroxylase (EC 1.14.13.1), which is covalently bound to glass beads and put into the reactor of a flow system. The enzyme catalyses the stoichiometric conversion of salicylic acid to catechol, which can then be detected amperometrically at + 0.45 V (vs. Ag/AgCl/KCl(sat.)). The electrode response is linearly proportional to the concentration of salicylic acid between 5 and 150 mug ml-1. The detection limit is 0.4 mug ml-1. This method is simple, rapid and specific. The assayed samples yielded relative standard deviations between 0.5 and 2.0% and recoveries between 93 and 98%.

引用

页码：469 / 475

页数：7

共 40 条

[1]

ALAN K, 1960, PEDIATRICS, V26, P800

[2] APPLICATION OF GAS-LIQUID-CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO ANALYSIS OF TRACE AMOUNTS OF SALICYLIC-ACID, ACETYLSALICYLIC ANHYDRIDE AND ACETYLSALICYLSALICYLIC ACID IN ASPIRIN SAMPLES AND ASPIRIN FORMULATIONS [J].