LPS-SENSITIVE PROTEASE ACTIVITY IN THE BLOOD-CELLS OF THE SOLITARY ASCIDIAN, CIONA-INTESTINALIS (L)

被引：5
|
作者：
JACKSON, AD [1 ]
SMITH, VJ [1 ]
机构：
[1] UNIV ST ANDREWS, SCH BIOL & MED SCI, GATTY MARINE LAB, ST ANDREWS KY16 8LB, FIFE, SCOTLAND
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 106卷 / 03期
关键词：
D O I：
10.1016/0305-0491(93)90124-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. LPS-sensitive protease activity in the blood cells of the solitary ascidian, Ciona intestinalis, was investigated in vitro. 2. Activity was found to be induced by the microbial carbohydrates, LPS and laminarin, but not by other sugars or carbohydrates. 3. Activity was effective against a broad range of chromogenic peptide substrates, dose-related, and inhibited by benzamidine, STI, PMSF, TPCK, TLCK and an organophosphorus insecticide, pirimiphosmethyl. 4. Activity induced by LPS treatment was short-lived and always preceded phenoloxidase activity in cell lysate supernatants.
引用
收藏
页码：505 / 512
页数：8
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