A COMPLEMENT-DEPENDENT ENZYME-IMMUNOASSAY (C-EIA) WITH INCREASED SENSITIVITY FOR IGM-RICH RABBIT SERA

被引：1
作者：
KUHN, HM [1 ]
BRADE, L [1 ]
BRADE, H [1 ]
机构：
[1] FORSCHUNGSINST BORSTEL, INST EXPTL BIOL & MED, DIV BIOCHEM MICROBIOL, PARKALLE 22, W-2061 BORSTEL, GERMANY
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 155卷 / 02期
关键词：
ENZYME IMMUNOASSAY; IGM ANTIBODY; RABBIT; COMPLEMENT; SENSITIVITY;
D O I：
10.1016/0022-1759(92)90286-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme immunoassay involving activation of complement (C-EIA) was developed for rabbit polyclonal IgM antibodies against lipid A and lipopolysaccharide antigens. C-EIA was significantly higher in sensitivity for IgM-rich rabbit sera compared to EIA using anti-immunoglobulin secondary antibodies. Hence, C-EIA should be useful for the detection of weak IgM reactivities in rabbit sera, especially after short-time immunizations. Selective inhibition of both complement pathways indicated that C-EIA measures activation of the classical pathway.
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收藏
页码：201 / 206
页数：6
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