INFLUENCE OF DNA ADENINE METHYLASE ON THE SENSITIVITY OF ESCHERICHIA-COLI TO NEAR-ULTRAVIOLET RADIATION AND HYDROGEN-PEROXIDE

被引：6

作者：

YALLALY, P ^{[1
]}

EISENSTARK, A ^{[1
]}

机构：

[1] CANC RES CTR,COLUMBIA,MO 65201

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91433-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Near-ultraviolet (NUV) radiation and hydrogen peroxide (H2O2) inactivation studies were performed on Escherichia coli K-12 DNA adenine methylation (dam) mutants and on cells that carry plasmids which overexpress Dam methylase. Lack of methylation resulted in increased sensitivity to NUV and H2O2 (a photoproduct of NUV). In a dam mutant carrying a dam plasmid, the levels of Dam enzyme and resistance to NUV and H2O2 were restored. However, using a multicopy dam+ plasmid strain, increasing the methylase above wildtype levels resulted in an increase in sensitivity of the cells rather than resistance. © 1990.

引用

页码：64 / 69

页数：6

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