INEFFICIENT DEGRADATION OF OXIDIZED REGIONS OF PROTEIN MOLECULES

被引：41
作者：
GRANT, AJ [1 ]
JESSUP, W [1 ]
DEAN, RT [1 ]
机构：
[1] HEART RES INST,145 MISSENDEN RD,CAMPERDOWN,SYDNEY 2050,AUSTRALIA
来源：
FREE RADICAL RESEARCH COMMUNICATIONS | 1993年 / 18卷 / 05期
关键词：
OXIDIZED PROTEIN; PROTEOLYSIS; HYDROLYSIS; MACROPHAGE; OXIDIZED; PROTEOLYTIC RESISTANCE;
D O I：
10.3109/10715769309147493
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have previously shown that the intracellular half-life of endocytosed oxidized albumin is much longer than that of native albumin.We now report that the regions of oxidized albumin which contain oxidation products (carbonyls and fluorophores), are less readily released as small degradation products by cell-free proteolysis than is the molecule overall. We deduce that oxidized moieties in the polypeptide chain can confer localized resistance to enzymatic proteolysis. Such resistance to proteolysis may account for the intracellular accumulation of some endocytosed oxidized protein which we have previously observed.
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收藏
页码：259 / 267
页数：9
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