INCREASED SHIKONIN PRODUCTION BY HAIRY ROOTS OF LITHOSPERMUM-ERYTHRORHIZON IN 2 PHASE BUBBLE COLUMN REACTOR

被引：33

作者：

SIM, SJ

CHANG, HN

机构：

[1] KOREA ADV INST SCI & TECHNOL,BIOPROC ENGN RES CTR,TAEJON 305701,SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant hairy root cultures of Lithospermum erythrorhizon were carried out to produce shikonin derivatives by employing in situ extraction with n-hexadecane in a shake flask and a bubble column bioreactor. Over 95 % shikonin produced was recovered in the n-hexadecane layer. In flask cultures the maximum concentration of shikonin with n-hexadecane extraction was 3 times higher than that obtained without extraction. In the two phase bubble column reactor, 572.6 mg/L of shikonin and 15.6 g/L of dry cell mass were obtained after 54 days. Shikonin was produced at a constant level of 10.6 mg/L.day during this period.

引用

页码：145 / 150

页数：6

共 35 条

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