DIFFERENTIAL-EFFECTS OF FLUORIDE AND A NONHYDROLYZABLE GTP ANALOG ON ADENYLATE-CYCLASE AND G-PROTEINS IN CERATITIS-CAPITATA NEURAL TISSUE

被引：1

作者：

GUILLEN, A ^{[1
]}

HOMBURGER, V ^{[1
]}

PEREZBAUN, JC ^{[1
]}

HARO, A ^{[1
]}

机构：

[1] CNRS,INSERM,CTR PHARMACOL ENDOCRINOL,F-34094 MONTPELLIER 5,FRANCE

来源：

CELLULAR SIGNALLING | 1993年 / 5卷 / 01期

关键词：

ADENYLATE CYCLASE; G-PROTEINS; ADP-RIBOSYLATION; FLUORIDE; GUANINE NUCLEOTIDES; INSECT NEURAL TISSUE;

D O I：

10.1016/0898-6568(93)90010-J

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have examined the effects of fluoride on guanine nucleotide-binding regulatory proteins (G-proteins) in neural membranes from the dipterous Ceratitis capitata. Fluoride effects on the G(s)-protein were monitored by determining adenylate cyclase activity and cholera toxin-catalysed ADP-ribosylation whereas those on the G(o)-protein were studied by measuring ADP-ribosylation with pertussis toxin. Data are discussed in relation to the effects of a non-hydrolysable GTP analogue. G-protein activation carried out by fluoride seems not to mimic, at least in insects, activation by non-hydrolysable GTP analogues, in opposition to that proposed for transducin, the G-protein of the mammalian visual system, and other G-proteins.

引用

页码：81 / 88

页数：8