CHIRAL SEPARATION OF DRUGS USING CYCLODEXTRINS IN CAPILLARY ZONE ELECTROPHORESIS

Chiral separation in capillary zone electrophoresis was investigated employing four kinds of cyclodextrins (CDs). The effects of the type and amount of CDs added to the background electrolyte and the pH of the buffer solution on the resolution of enantiomers were examined. The best enantioselectivity was obtained by employing heptakis(2,6-di-O-methyl)-beta-cyclodextrin in acidic solution. In particular, the enantiomeric separation of denopamine, which is a potent cardiotonic agent, the direct enantiomeric separation of which had not been successful, was achieved by the method. The effects of buffer, urea and an organic solvent on the enantioselectivity of three enantiomeric drugs were also investigated.