TRANSFORMATION OF VICIA-NARBONENSIS VIA AGROBACTERIUM-MEDIATED GENE-TRANSFER

被引：1
作者：
PICKARDT, T
MEIXNER, M
SCHADE, V
SCHIEDER, O
机构：
来源：
PLANT CELL REPORTS | 1991年 / 9卷 / 10期
关键词：
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/1 hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopalin-positive somatic embroyos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.
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页码：535 / 538
页数：4
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