DETECTION AND CHARACTERIZATION OF APOPTOSIS IN OSTEOCLASTS IN-VITRO

被引：62

作者：

KAMEDA, T

ISHIKAWA, H

TSUTSUI, T

机构：

[1] NIPPON DENT UNIV TOKYO, SCH DENT, DEPT PHARMACOL, CHIYODA KU, TOKYO 102, JAPAN

[2] NIPPON DENT UNIV TOKYO, SCH DENT, DEPT ORTHODONT, CHIYODA KU, TOKYO 102, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Morphological changes of nuclei and DNA fragmentation undergoing cell death were studied using highly purified rabbit osteoclasts in vitro. Approximately fifty percent of dead osteoclasts exhibited morphological changes of nuclei characterized by apoptosis and similar to 70% of osteoclasts with changes of nuclei exhibited DNA fragmentation in our culture system. These changes of nuclei were confirmed as condensation of chromatin through transmission electronmicroscope. Moreover, biochemical characterizations of these changes were compatible with the characterizations of apoptosis in other cell systems. These results demonstrate that part of osteoclasts die by a mode of apoptosis. (C) 1995 Academic Press, Inc.

引用

页码：753 / 760

页数：8

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