EFFECTS OF FETAL CALF SERUM ON CELL VIABILITY, CYTOTOXICITY AND DETOXIFICATION IN THE 2 KIDNEY-DERIVED CELL-LINES LLC-PK1 AND MDCK

被引：18

作者：

BOHETS, HH ^{[1
]}

NOUWEN, EJ ^{[1
]}

DEBROE, ME ^{[1
]}

DIERICKX, PJ ^{[1
]}

机构：

[1] UNIV ANTWERP,DEPT NEPHROL,B-2650 ANTWERP,BELGIUM

来源：

TOXICOLOGY IN VITRO | 1994年 / 8卷 / 04期

关键词：

D O I：

10.1016/0887-2333(94)90016-7

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Foetal calf serum (FCS) dependent cell viability, cytotoxicity and detoxification were investigated in MDCK and LLC-PK1 cells. FCS was used at 0-10% (v/v). Viability and cytotoxicity were measured by neutral red uptake and by the MTT test. Viability of LLC-PK1 was strongly dependent on the FCS concentration, but that of MDCK cells only to a very limited extent. For both cell lines the cytotoxicity of HgCl2 was FCS concentration dependent: lower toxicity was observed with 5-10% FCS than with 0-1% FCS. This effect was not observed for paracetamol. The results could not be explained by altered glutathione or glutathione S-transferase. The optimal FCS concentration of 1%, necessary to retain cell viability, had a limited influence on cytotoxicity. FCS concentration must be taken into consideration when cytotoxicity data from different studies are compared.

引用

页码：559 / 561

页数：3