MICROTITRE PLATE ENZYME AMPLIFIED IMMUNOASSAY FOR THYROID STIMULATING HORMONE

被引：2

作者：

WILSON, R

机构：

[1] Research Centre for Advanced Science and Technology, University of Tokyo, Tokyo

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 269卷 / 02期

基金：

日本学术振兴会;

关键词：

IMMUNOASSAY; ENZYME AMPLIFIED IMMUNOASSAY; FLAVIN ADENINE DINUCLEOTIDE PHOSPHATE; MICROTITRE PLATE; NITROETHANE; SUICIDE SUBSTRATE; THYROID STIMULATING HORMONE;

D O I：

10.1016/0003-2670(92)85416-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Methods for removing contaminating FAD and alkaline phosphatase from apo-aminoacid oxidase are described. A method for phosphorylating flavin adenine dinucleotide is also described. Its hydrolysis to flavin adenine dinucleotide catalysed by alkaline phosphatase is demonstrated by liquid chromatography. Flavin adenine dinucleotide can reactivate apo-aminoacid oxidase and result in the formation of hydrogen peroxide. The hydrogen peroxide can be detected colorimetrically. These reactions are used in an enzyme amplified immunoassay for thyroid stimulating hormone in which alkaline phosphatase is the enzyme label. A limit of detection of 0.03 muIU ml-1 for thyroid stimulating hormone makes the assay suitable for diagnosing hyperthyroidism.

引用

页码：301 / 306

页数：6

共 17 条

[1]

ALSTON TA, 1983, J BIOL CHEM, V258, P1136

[2] A SUBSTRATE AMPLIFICATION SYSTEM FOR ENZYME-LINKED IMMUNOASSAYS - DEMONSTRATION OF ITS GENERAL APPLICABILITY TO ELISA SYSTEMS FOR DETECTING ANTIBODIES AND IMMUNE-COMPLEXES [J].