IMMUNOELECTRON MICROSCOPIC DISTRIBUTION OF HISTONE-H2B AND HISTONE-H3 AND PROTAMINES IN THE COURSE OF MOUSE SPERMIOGENESIS

被引:26
|
作者
BIGGIOGERA, M
MULLER, S
COURTENS, JL
FAKAN, S
ROMANINI, MGM
机构
[1] INRA, PHYSIOL REPROD STN, F-37380 MONNAIE, FRANCE
[2] CNRS, INST BIOL MOLEC & CELLULAIRE, F-67084 STRASBOURG, FRANCE
[3] UNIV PAVIA, DIPARTMENTO BIOL ANIM, I-27100 PAVIA, ITALY
关键词
ULTRASTRUCTURE; IMMUNOCYTOCHEMISTRY; CHROMATIN STRUCTURE; NUCLEAR PROTEINS; TESTIS;
D O I
10.1002/jemt.1070200305
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
We have followed the fine structural distribution of two nucleosomal core histones, H2B and H3, and of protamines in the course of mouse spermiogenesis by means of specific antibodies and ultrastructural immunocytochemistry. Our results demonstrate that the nuclear labeling density of histone H2B decreases during steps 6-8 and then increases again in step 9-10 spermatids, while the labeling for histone H3 is constant throughout this period. In step 12 spermatids, the anti-H2B antibody labels mainly the central area of the nucleus. The first signs of protamine labeling are present in step 12 spermatids, where the gold grains can be found over the periphery of the nucleus. Later on, protamine labeling constantly increases and, by the end of spermiogenesis, the whole nucleus is labeled. We suggest that the morphological and structural differences between the central area and the periphery of mouse spermatids are, at least partly, due to a difference in the protein moiety associated with DNA. The central area, which is peculiar to the mouse and has been previously considered as a focus of chromatin condensation, represents, however, the last nuclear region containing histones and consequently the last area where the substitution of histones by protamines takes place.
引用
收藏
页码:259 / 267
页数:9
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