DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN BREAST-MILK BY MEANS OF POLYMERASE CHAIN-REACTION

被引：26
作者：
HOTSUBO, T
NAGATA, N
SHIMADA, M
YOSHIDA, K
FUJINAGA, K
CHIBA, S
机构：
[1] SAPPORO MED UNIV,SCH MED,CANC RES INST,DEPT MOLEC BIOL,SAPPORO,HOKKAIDO 060,JAPAN
[2] TAKARA SHUZO CO LTD,BIO RES LABS,OTSU,SHIGA 520,JAPAN
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 10期
关键词：
HUMAN CYTOMEGALOVIRUS; BREAST MILK; POLYMERASE CHAIN REACTION;
D O I：
10.1111/j.1348-0421.1994.tb01862.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.
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页码：809 / 811
页数：3
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