DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN BREAST-MILK BY MEANS OF POLYMERASE CHAIN-REACTION

被引：26

作者：

HOTSUBO, T

NAGATA, N

SHIMADA, M

YOSHIDA, K

FUJINAGA, K

CHIBA, S

机构：

[1] SAPPORO MED UNIV,SCH MED,CANC RES INST,DEPT MOLEC BIOL,SAPPORO,HOKKAIDO 060,JAPAN

[2] TAKARA SHUZO CO LTD,BIO RES LABS,OTSU,SHIGA 520,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 10期

关键词：

HUMAN CYTOMEGALOVIRUS; BREAST MILK; POLYMERASE CHAIN REACTION;

D O I：

10.1111/j.1348-0421.1994.tb01862.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.

引用

页码：809 / 811

页数：3

共 11 条

[1] CYTOMEGALO-VIRUS IN BREAST-MILK OF SWEDISH MILK DONORS [J].