TRANSCRIPTIONAL ACTIVATION BY TETRACYCLINES IN MAMMALIAN-CELLS

被引：1991

作者：

GOSSEN, M ^{[1
]}

FREUNDLIEB, S ^{[1
]}

BENDER, G ^{[1
]}

MULLER, G ^{[1
]}

HILLEN, W ^{[1
]}

BUJARD, H ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG, INST MIKROBIOL & BIOCHEM, D-91058 ERLANGEN, GERMANY

来源：

SCIENCE | 1995年 / 268卷 / 5218期

关键词：

D O I：

10.1126/science.7792603

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A transcriptional transactivator was developed that fuses the VP16 activation domain with a mutant Tet repressor from Escherichia coli. This transactivator requires certain tetracycline (Tc) derivatives for specific DNA binding. Thus, addition of doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold. The specificity of the Tet repressor-operator-effector interaction and the pharmacological characteristics of Tc's make this regulatory system well suited for the control of gene activities in vivo, such as in transgenic animals and possibly in gene therapy.

引用

页码：1766 / 1769

页数：4

共 16 条

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