REAGENTS FOR THE SITE-SPECIFIC CLEAVAGE OF MEGABASE DNA

被引：71

作者：

DERVAN, PB

机构：

来源：

NATURE | 1992年 / 359卷 / 6390期

关键词：

D O I：

10.1038/359087a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The physical mapping of chromosomes will be facilitated by methods of breaking large DNA into manageable fragments, or cutting uniquely at genetic markers of interest. Key issues in the design of sequence-specific DNA cleaving reagents are the specificity of binding, the number of different sequences that can be targeted and the cleavage yield.

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页码：87 / 88

页数：2

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