CLONING AND EXPRESSION REGULATION OF LYSA GENE OF BACILLUS-SUBTILIS

被引:0
作者
KALCHEVA, EO
SHANSKAYA, VO
MALUTA, SS
机构
来源
GENETIKA | 1992年 / 28卷 / 05期
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中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cloning of Bacillus subtilis DNA fragment with the lysA gene encoding diaminopimelatecarboxilase (EC 4.1.1.20) was done. The cloned gene in poorly expressed both in Escherichia coli and in Bacillus subtilis. Some DNA sequence distanced from the lysA gene seems to be necessary for full gene expression, this sequence having been not cloned together with the lysA. The sequence in needed for regulation of the expression as well.
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页码:5 / 10
页数:6
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