A NEW WAY OF BIOSENSING USING FLUORESCENCE ENERGY-TRANSFER AND LANGMUIR-BLODGETT-FILMS

被引：4
作者：
SIEGMUND, HU
BECKER, A
机构：
[1] Bayer AG, Zentrale Forschung Uerdingen
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 1993年 / 11卷 / 1-3期
关键词：
D O I：
10.1016/0925-4005(93)85244-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method of detecting the binding of ligand molecules (mannosides) to a biological receptor (Con A) by Forster energy transfer from Langmuir-Blodgett (LB) films is presented. The LB films consist of pre-polymerized amphiphilic polymethacrylates containing a coumarin fluorophore (donor) and reactive groups to which biological receptors can be attached. Studies determining the stability of the system are described. The specific binding of rhodamine (acceptor)-labelled ligands and their competitive replacement by unlabelled analytes is demonstrated. A simple dual-channel fluorescence detector optimized for this purpose is described as well.
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页码：103 / 108
页数：6
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