CONSERVATION OF INFECTIVITY IN PURIFIED FIBRILLARY EXTRACTS OF SCRAPIE-INFECTED HAMSTER BRAIN AFTER SEQUENTIAL ENZYMATIC DIGESTION OR POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：48
作者：
BROWN, P
LIBERSKI, PP
WOLFF, A
GAJDUSEK, DC
机构：
[1] Central Nervous Sys. Lab., Natl. Inst. Neuro. Disorders, National Institutes of Health, Bethesda
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 18期
关键词：
enzymes; scrapie amyloid protein; scrapie-associated fibrils;
D O I：
10.1073/pnas.87.18.7240
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.
引用
收藏
页码：7240 / 7244
页数：5
相关论文
共 49 条
[1]   STUDIES ON DNA FROM NORMAL AND SCRAPIE-AFFECTED MOUSE BRAIN [J].
ADAMS, DH .
JOURNAL OF NEUROCHEMISTRY, 1972, 19 (08) :1869-&
[2]   DOES AGENT OF SCRAPIE REPLICATE WITHOUT NUCLEIC ACID [J].
ALPER, T ;
CRAMP, WA ;
HAIG, DA ;
CLARKE, MC .
NATURE, 1967, 214 (5090) :764-&
[3]   PURIFIED SCRAPIE PRIONS RESIST INACTIVATION BY PROCEDURES THAT HYDROLYZE, MODIFY, OR SHEAR NUCLEIC-ACIDS [J].
BELLINGERKAWAHARA, C ;
DIENER, TO ;
MCKINLEY, MP ;
GROTH, DF ;
SMITH, DR ;
PRUSINER, SB .
VIROLOGY, 1987, 160 (01) :271-274
[4]   PURIFIED SCRAPIE PRIONS RESIST INACTIVATION BY UV IRRADIATION [J].
BELLINGERKAWAHARA, C ;
CLEAVER, JE ;
DIENER, TO ;
PRUSINER, SB .
JOURNAL OF VIROLOGY, 1987, 61 (01) :159-166
[5]   PURIFICATION AND PARTIAL CHARACTERIZATION OF THE NORMAL CELLULAR HOMOLOG OF THE SCRAPIE AGENT PROTEIN [J].
BENDHEIM, PE ;
POTEMPSKA, A ;
KASCSAK, RJ ;
BOLTON, DC .
JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (06) :1198-1208
[6]   ISOLATION AND STRUCTURAL STUDIES OF THE INTACT SCRAPIE AGENT PROTEIN [J].
BOLTON, DC ;
BENDHEIM, PE ;
MARMORSTEIN, AD ;
POTEMPSKA, A .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 258 (02) :579-590
[7]   IDENTIFICATION OF A PROTEIN THAT PURIFIES WITH THE SCRAPIE PRION [J].
BOLTON, DC ;
MCKINLEY, MP ;
PRUSINER, SB .
SCIENCE, 1982, 218 (4579) :1309-1311
[8]   SCRAPIE AND CELLULAR PRION PROTEINS DIFFER IN THEIR KINETICS OF SYNTHESIS AND TOPOLOGY IN CULTURED-CELLS [J].
BORCHELT, DR ;
SCOTT, M ;
TARABOULOS, A ;
STAHL, N ;
PRUSINER, SB .
JOURNAL OF CELL BIOLOGY, 1990, 110 (03) :743-752
[9]   INABILITY TO TRANSMIT SCRAPIE BY TRANSFECTION OF MOUSE EMBRYO CELLS-INVITRO [J].
BORRAS, MT ;
KINGSBURY, DT ;
GAJDUSEK, DC ;
GIBBS, CJ .
JOURNAL OF GENERAL VIROLOGY, 1982, 58 (FEB) :263-271
[10]   MOLECULAR HYBRIDIZATION STUDIES WITH SCRAPIE BRAIN NUCLEIC-ACIDS .2. DIFFERENTIAL EXPRESSION IN SCRAPIE HAMSTER BRAIN [J].
BORRAS, T ;
MERENDINO, JJ ;
GIBBS, CJ .
ARCHIVES OF VIROLOGY, 1986, 88 (1-2) :79-90
12345