IDENTIFICATION OF MYCOPLASMA-HYOPNEUMONIAE WITH A DNA PROBE

被引：11
|
作者：
AHRENS, P
FRIIS, NF
机构：
[1] National Veterinary Laboratory, Copenhagen, DK-1503
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 12卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1991.tb00551.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown organisms. The S-35-labelled probe could detect as little as 100 pg of DNA or 10(5) colour changing units. This is a possible alternative diagnostic procedure for enzootic pneumonia of pigs.
引用
收藏
页码：249 / 253
页数：5
相关论文
共 50 条
  • [11] INVITRO SUSCEPTIBILITY OF MYCOPLASMA-HYOPNEUMONIAE TO ANTIBIOTICS
    YAMAMOTO, K
    KOSHIMIZU, K
    OGATA, M
    JAPANESE JOURNAL OF VETERINARY SCIENCE, 1986, 48 (01): : 1 - 5
  • [12] A GENE PROBE TO DETECT MYCOPLASMA-HYOPNEUMONIAE, THE ETIOLOGICAL AGENT OF ENZOOTIC PORCINE PNEUMONIA
    STEMKE, GW
    MOLECULAR AND CELLULAR PROBES, 1989, 3 (03) : 225 - 232
  • [13] SERUM AND MUCOSAL ANTIBODY-RESPONSES AGAINST MYCOPLASMA-HYOPNEUMONIAE FOLLOWING INTRAPERITONEAL VACCINATION AND CHALLENGE OF PIGS WITH MYCOPLASMA-HYOPNEUMONIAE
    SHELDRAKE, RF
    ROMALIS, LF
    SAUNDERS, MM
    RESEARCH IN VETERINARY SCIENCE, 1993, 55 (03) : 371 - 376
  • [14] COMPARISON OF MYCOPLASMA-HYOPNEUMONIAE STRAINS BY SEROLOGIC METHODS
    RO, LH
    ROSS, RF
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1983, 44 (11) : 2087 - 2094
  • [15] A NEW, INEXPENSIVE MEDIUM FOR CULTIVATION OF MYCOPLASMA-HYOPNEUMONIAE
    HO, CL
    CHU, TH
    CHIN, HS
    REVIEWS OF INFECTIOUS DISEASES, 1982, 4 : S265 - S265
  • [16] INVITRO COLONIZATION OF PORCINE TRACHEA BY MYCOPLASMA-HYOPNEUMONIAE
    JACQUES, M
    BLANCHARD, B
    FOIRY, B
    GIRARD, C
    KOBISCH, M
    ANNALES DE RECHERCHES VETERINAIRES, 1992, 23 (03): : 239 - 247
  • [17] COMPARISON OF MYCOPLASMA-HYOPNEUMONIAE STRAINS BY SEROLOGIC METHODS
    RO, LH
    ROSS, RF
    YALE JOURNAL OF BIOLOGY AND MEDICINE, 1983, 56 (5-6): : 925 - 927
  • [18] DURATION OF MYCOPLASMA-HYOPNEUMONIAE INFECTION IN GNOTOBIOTIC PIGS
    UNDERDAHL, NR
    KENNEDY, GA
    RAMOS, AS
    CANADIAN VETERINARY JOURNAL-REVUE VETERINAIRE CANADIENNE, 1980, 21 (09): : 258 - 261
  • [19] INTERACTIONS OF MYCOPLASMA-HYOPNEUMONIAE MEMBRANES WITH PORCINE LYMPHOCYTES
    MESSIER, S
    ROSS, RF
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1991, 52 (09) : 1497 - 1502
  • [20] A NOVEL REPETITIVE SEQUENCE FROM MYCOPLASMA-HYOPNEUMONIAE
    HARASAWA, R
    ASADA, K
    KATO, I
    JOURNAL OF VETERINARY MEDICAL SCIENCE, 1995, 57 (03): : 557 - 558
12345