IDENTIFICATION OF MYCOPLASMA-HYOPNEUMONIAE WITH A DNA PROBE

被引：11
|
作者：
AHRENS, P
FRIIS, NF
机构：
[1] National Veterinary Laboratory, Copenhagen, DK-1503
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 12卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1991.tb00551.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown organisms. The S-35-labelled probe could detect as little as 100 pg of DNA or 10(5) colour changing units. This is a possible alternative diagnostic procedure for enzootic pneumonia of pigs.
引用
收藏
页码：249 / 253
页数：5
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