PHYSIOLOGICAL CHARACTERIZATION OF A CADMIUM-RESISTANT MUTANT IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE

被引：5
作者：
WUNDERLICH, C [1 ]
ZHAO, QS [1 ]
ZIMMERMANN, M [1 ]
WOLF, K [1 ]
机构：
[1] RHEIN WESTFAL TH AACHEN,INST BIOL 4 MIKROBIOL,D-52056 AACHEN,GERMANY
来源：
MICROBIOLOGICAL RESEARCH | 1995年 / 150卷 / 03期
关键词：
SCHIZOSACCHAROMYCES POMBE; FISSION YEAST; HEAVY METALS; CADMIUM;
D O I：
10.1016/S0944-5013(11)80002-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
After treatment of the wild-type strain 975 h(+) with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutants able to grow on YEP-medium containing 15 mM CdCl2 were isolated. Mutant R17 showed a specific resistance to Cd, but no increased tolerance to Pb(NO3)(2), CuSO4 or ZnCl2. No higher content of Cd-binding phytochelatins could be detected, but the mutant accumulated lower levels of Cd than the corresponding wild-type. No change, however, was observed in the accumulation of Cu. Under the influence of Cd, the respiratory activity of mutant R17 is reduced less than in the wild-type. The mechanism of resistance could be an increased secretion or a decreased uptake of Cd.
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页码：233 / 237
页数：5
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