SURFACE-PLASMON STUDIES OF SPECIFIC RECOGNITION REACTIONS AT SELF-ASSEMBLED MONOLAYERS ON GOLD

被引:39
|
作者
SCHMITT, FJ [1 ]
HAUSSLING, L [1 ]
RINGSDORF, H [1 ]
KNOLL, W [1 ]
机构
[1] UNIV MAINZ,INST ORGAN CHEM,W-6500 MAINZ,GERMANY
关键词
D O I
10.1016/0040-6090(92)90412-5
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Specific recognition reactions of proteins at functionalized interfaces were observed with plasmon surface polariton (PSP) spectroscopy. This optical method offers a vertical resolution of about 0.2 nm in aqueous media. Different types of receptor surfaces were prepared, either by self-assembly of biotinylated alkane thiols or by specific adsorption of streptavidin monolayers. The corresponding substrates were streptavidin and a biotinylated monoclonal antibody. Their binding properties are inversely related to the packing density of the receptors. A good accessibility is only given in the case of a "diluted" presentation of the receptors at the interface. Both binding site and diffusion limited adsorption have been observed.
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页码:815 / 817
页数:3
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