CHROMOSOME PAINTING USING CHROMOSOME-SPECIFIC PROBES FROM FLOW-SORTED PIG CHROMOSOMES

被引：19
作者：
LANGFORD, CF
TELENIUS, H
CARTER, NP
MILLER, NGA
TUCKER, EM
机构：
[1] AFRC,INST ANIM PHYSIOL & GENET RES,CAMBRIDGE RES STN,CAMBRIDGE CB2 4AT,ENGLAND
[2] KAROLINSKA INST,DEPT CLIN GENET,S-10401 STOCKHOLM 60,SWEDEN
[3] UNIV CAMBRIDGE,DEPT PATHOL,CAMBRIDGE,ENGLAND
来源：
CYTOGENETICS AND CELL GENETICS | 1992年 / 61卷 / 03期
关键词：
D O I：
10.1159/000133411
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Biotinylated chromosome-specific probes were prepared from flow-sorted pig chromosomes 1, 13, 18, X, and Y using the degenerate oligonucleotide-primed polymerase chain reaction. Probes prepared in this way can be used to confirm the identity of chromosomes in the bivariate pig flow karyotype and in pig x mouse somatic cell hybrids.
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页码：221 / 223
页数：3
相关论文
共 10 条
[1]  
ARCHIBALD A, 1991, ANIM GENET S1, V22, P82
[2]  
DIXON SC, 1992, ANIM GENET, V23, P203, DOI 10.1111/j.1365-2052.1992.tb00132.x
[3]   SWINE CHROMOSOMES - FLOW SORTING AND SPOT BLOT HYBRIDIZATION [J].
GRUNWALD, D ;
GEFFROTIN, C ;
CHARDON, P ;
FRELAT, G ;
VAIMAN, M .
CYTOMETRY, 1986, 7 (06) :582-588
[4]   RAPID DETECTION OF HUMAN CHROMOSOME-21 ABERRATIONS BY INSITU HYBRIDIZATION [J].
LICHTER, P ;
CREMER, T ;
TANG, CC ;
WATKINS, PC ;
MANUELIDIS, L ;
WARD, DC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9664-9668
[5]  
MILLER JR, 1992, IN PRESS CYTOGENET C
[6]   CYTOGENETIC ANALYSIS USING QUANTITATIVE, HIGH-SENSITIVITY, FLUORESCENCE HYBRIDIZATION [J].
PINKEL, D ;
STRAUME, T ;
GRAY, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (09) :2934-2938
[7]   FLUORESCENCE INSITU HYBRIDIZATION WITH HUMAN CHROMOSOME-SPECIFIC LIBRARIES - DETECTION OF TRISOMY-21 AND TRANSLOCATIONS OF CHROMOSOME-4 [J].
PINKEL, D ;
LANDEGENT, J ;
COLLINS, C ;
FUSCOE, J ;
SEGRAVES, R ;
LUCAS, J ;
GRAY, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9138-9142
[8]   DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR - GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER [J].
TELENIUS, H ;
CARTER, NP ;
BEBB, CE ;
NORDENSKJOLD, M ;
PONDER, BAJ ;
TUNNACLIFFE, A .
GENOMICS, 1992, 13 (03) :718-725
[9]   CYTOGENETIC ANALYSIS BY CHROMOSOME PAINTING USING DOP-PCR AMPLIFIED FLOW-SORTED CHROMOSOMES [J].
TELENIUS, H ;
PELMEAR, AH ;
TUNNACLIFFE, A ;
CARTER, NP ;
BEHMEL, A ;
FERGUSONSMITH, MA ;
NORDENSKJOLD, M ;
PFRAGNER, R ;
PONDER, BAJ .
GENES CHROMOSOMES & CANCER, 1992, 4 (03) :257-263
[10]  
VERMA RS, HUMAN CHROMOSOMES MA, P115
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