IDENTIFICATION OF THE SURFACE POLYPEPTIDE ANTIGENS OF THE INFECTIVE LARVAE OF ONCHOCERCA-VOLVULUS

被引：2

作者：

TITANJI, VPK

MBACHAM, WF

机构：

[1] The Biotechnology Center, University of Yaoundé, Yaoundé

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 84卷 / 05期

关键词：

D O I：

10.1016/0035-9203(90)90153-6

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The surface polypeptide antigens of third-stage infective larvae (L3) and adult males of Onchocerca volvulus have been compared after iodogen-catalysed radioiodination, separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in I-mercaptoethanol followed by autoradiography. L3 surfaces contained polypeptides with apparent molecular weights of 14, 18A 5, 26, 34, 51, 68 and 130 kDa, whereas the adults contained 14, 19, 26, 34, 37A 5 and 68 kDa components. By immunoprecipitation with patient's sera, only the 14 and 18A 5 kDa components were shown to be antigenic on the L3, the other components being unreactiye with patients' antibodies. Under similar conditions, 4 of the 6 adult male polypeptides reacted with patients' antisera, confirming their antigenic nature. Lentil lectin and immunosorbent chromatography of the surface components revealed the 18A 5 kDa and 68 kDa antigens of L3 and adult males respectively to be glycoproteins. The apparently weak reactivity of L3 surface components with host antibody may be part of an escape mechanism that favours the establishment of O. volvulus in human hosts. © 1990, Royal Society of Tropical Medicine and Hygiene. All Rights Reserved.

引用

页码：696 / 700

页数：5

共 17 条

[1] COMPARISON OF SURFACE IODINATION METHODS BY ELECTRON-MICROSCOPIC AUTORADIOGRAPHY APPLIED INVITRO TO DIFFERENT LIFE-STAGES OF DIPETALONEMA-VITEAE (FILARIOIDEA) [J].