FLUOROMETRIC SUBSTRATE FOR SULFATASE AND LIPASE

被引：59

作者：

GUILBAULT, GG

HIESERMAN, J

机构：

[1] Department of Chemistry, Louisiana State University, Lakefront Campus, New Orleans

来源：

ANALYTICAL CHEMISTRY | 1969年 / 41卷 / 14期

关键词：

D O I：

10.1021/ac50159a034

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

New fluorometric substrates are described for the assay of the enzyme sulfatase and lipase. β-Naphthol sulfate and 4-methyl umbelllferone sulfate are useful for the assay of sulfatases and N-methyl indoxyl myristate is an excellent substrate for the analysis of lipase. As little as 10-4 unit per ml of lipase or 10-3 unit per ml of sulfatase can be determined by a direct reaction rate method in 2-3 minutes with a precision and accuracy of about 1.5%. © 1969, American Chemical Society. All rights reserved.

引用

页码：2006 / +

页数：1

共 15 条

[1] STUDIES ON SULPHATASES .4. ARYLSULPHATASE AND BETA-GLUCURONIDASE CONCENTRATES FROM LIMPETS [J].