Validated, reversed phase high performance liquid chromatography method for the estimation of atazanavir sulfate in pharmaceutical formulations

Reversed phase high performance liquid chromatographic method was developed and validated for estimation of Atazanavir Sulfate in tablet dosage form. A Sunfire C18, 250x4.6 mm i.d, 5 mu m partical size, with mobile phase consisting of a buffer of 0.05 M ammonium dihydrogen orthophosphate in water and acetonitrile in the ratio of 30: 70 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 252 nm. The retention time was 4.78min. The detector response was linear in the concentration of 80-240mcg/ml, with the regression coefficient of 0.9999. Quantification was done by calculating area of the peak and the detection and quantitation limits were 0.04 and 0.12 mcg/ml respectively. The percentage assay of Atazanavir Sulfate was 98.73%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method can be applied for the determination of Atazanavir Sulfate in quality control samples and formulations without interferences of the excipients present.