PLANT-REGENERATION FROM CALLUS AND PROTOPLAST CULTURES OF LOTUS-PEDUNCULATUS CAV

被引：2
作者：
PUPILLI, F
ARCIONI, S
DAMIANI, F
PEZZOTTI, M
机构：
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 23卷 / 03期
关键词：
FORAGE LEGUMES; LOTUS-PEDUNCULATUS; PLANT REGENERATION; PROTOPLAST CULTURE;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Plant regeneration from leaf- and cotyledon-derived calli and from protoplast-derived tissue has been obtained in Lotus pedunculatus. Callus induction was achieved with 2,4-D and plant regeneration required the following two media sequences: bud formation was stimulated by IAA and BA and shoot growth by kinetin. Root formation occurred in the presence of IAA. Cotyledon protoplasts showed a low plating efficiency and plant regeneration was achieved via an intervening callus phase.
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页码：193 / 199
页数：7
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