OPTIMIZATION OF GLASS MICROELECTRODE PROPERTIES BY RESPONSE-SURFACE METHODOLOGY

被引：2

作者：

KISAALITA, WS

SKEEN, RS

VANWIE, BJ

BARNES, CD

FUNG, SJ

机构：

[1] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164

[2] WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT,PULLMAN,WA 99164

[3] WASHINGTON STATE UNIV,DEPT PHARMACOL & PHYSIOL,PULLMAN,WA 99164

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1991年 / 40卷 / 2-3期

基金：

美国国家科学基金会;

关键词：

GLASS MICROELECTRODE; INTRACELLULAR RECORDING AND STIMULATION; MICROPIPETTE PULLER; EXPERIMENTAL FACTORIAL DESIGN; SURFACE RESPONSE METHODOLOGY;

D O I：

10.1016/0165-0270(91)90059-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glass microelectrodes filled with electrolyte solutions are standard tools for electrophysiological studies. However, for any given application, there are limitations to the properties of the microelectrode, such as impedance and shank length, that can yield satisfactory results. The trial and error approach in pulling electrodes with the desired properties can be time consuming. The use of a response surface procedure which allows the experimenter to change more than one factor at a time and therefore determine the desired puller condition more efficiently is demonstrated. Also, design improvements for the World Precision Instrument, Model PUL-1, Microelectrode puller, used in this study are suggested.

引用

页码：113 / 120

页数：8

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