The grain, flour and dough quality of the octoploid tritordeum, amphiploid derived from the cross Hordeum chilense Roem. et Schulz. x Triticum aestivum L em. Thell., were studied in comparison with those of durum and bread wheats, and hexaploid tritordeum (H. chilense x T turgidum conv. durum Desf. em. M.K.). Small-scale tests, commonly used for evaluating quality in cereals, together with the alveograph, were performed. Both hexa- and octoploid tritordeum exhibited quality characteristics similar to those of bread wheat and very different from that durum wheat. Nevertheless, some genetic differences were found between hexa- and octoploid tritordeum and between the different genetic lines used of each one. This genetic variability opens the possibility that both hexa- and octoploid tritordeum could be used as new genetic sources in these kind of characters.