A SIMPLE MOST PROBABLE NUMBER TECHNIQUE FOR THE SENSITIVE RECOVERY OF A GENETICALLY-MODIFIED PSEUDOMONAS-AUREOFACIENS FROM SOIL

被引：18

作者：

DELEIJ, FAAM ^{[1
]}

BAILEY, MJ ^{[1
]}

WHIPPS, JM ^{[1
]}

LYNCH, JM ^{[1
]}

机构：

[1] INST VIROL & ENVIRONM MICROBIOL,MOLEC MICROBIAL ECOL LAB,NAT ENVIRONM RES COUNCIL,OXFORD OX1 35R,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 16卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb00364.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strain of Pseudomonas aureofaciens (SBW25EeZY-6KX) that was chromosomally marked with a lacZY and a km(r)-xylE cassette could be recovered from non-sterile soil by a selective Pseudomonas enrichment broth amended with 100 ppm kanamycin and 50 ppm X-gal in an MPN (most probable number) assay. The assay was sensitive and reliable, allowing detection of as few as one recombinant cell in a 1% (w/v) soil suspension. The soil used contained a large (ca 5% of total culturable bacteria) background of indigenous bacteria that were either able to utilize lactose, were resistant to kanamycin, or both.

引用

页码：307 / 310

页数：4

共 7 条

[1] A BROAD-HOST-RANGE SHUTTLE SYSTEM FOR GENE INSERTION INTO THE CHROMOSOMES OF GRAM-NEGATIVE BACTERIA [J].