PH AND BUFFERING IN THE BICINCHONINIC ACID (4,4'-DICARBOXY-2,2'-BIQUINOLINE) PROTEIN ASSAY

被引：13

作者：

ZHANG, JX ^{[1
]}

HALLING, PJ ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,DEPT BIOSCI & BIOTECHNOL,GLASGOW G1 1XW,SCOTLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 188卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90520-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The HCO3-/CO32- buffer used in the bicinchoninic acid (BCA) protein assay has only weak buffering capacity at the recommended pH (11.25). Consequently the assay is rather sensitive to interference from effectively acid or alkaline samples, particularly in the micro method. Adjustment of pH in these alkaline solutions of high [Na+] is complicated by Na+ errors on the pH electrode. Hence it is recommended to prepare the buffers from known amounts of NaHCO3 and Na2CO3, and to reduce the pH to around 10.7; this offers much better buffering capacity with only a limited reduction in color development. © 1990.

引用

页码：9 / 10

页数：2