STRUCTURE-FUNCTION-RELATIONSHIPS OF MITOCHONDRIAL MONOAMINE-OXIDASE-A AND MONOAMINE-OXIDASE-B CHIMERIC FORMS

被引：39
作者：
GOTTOWIK, J [1 ]
MALHERBE, P [1 ]
LANG, G [1 ]
DAPRADA, M [1 ]
CESURA, AM [1 ]
机构：
[1] F HOFFMANN LA ROCHE & CO LTD, NERVOUS SYST DIS, PRECLIN RES, DIV PHARMA, CH-4002 BASEL, SWITZERLAND
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期
关键词：
MONOAMINE OXIDASE; CHIMERIC ENZYME; STRUCTURE FUNCTION RELATIONSHIPS; LAZABEMIDE;
D O I：
10.1111/j.1432-1033.1995.tb20639.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Monoamine oxidases (MAO) A and B show a high degree of amino acid similarity. Apart from the NH2-terminus, which contains an ADP-binding consensus sequence, little is known about their structural features or the sequences involved in the binding of substrates. In the present paper, we have studied the structure/function relationships of MAOs by constructing 18 different chimeric forms of MAO, engineered by moving progressively the junction between the NH2-terminus of one MAO form with the COOH-terminus of its isoenzyme. After transient expression in HEK-293 cells, the properties of these chimeric enzymes were investigated using both selective and nonselective substrates and inhibitors. Whereas exchange of the ADP-binding sequence did not modify the catalytic properties of either MAO isoforms, chimeras with increasing length of the NH2-terminus of MAO-A (up to residue 256) showed a marked decrease in affinity towards the MAO-B substrate phenylethylamine and the inhibitor N-(2-aminoethyl)-5-chloro-2-pyridine carboxamide . HCl (lazabemide) when compared to wild-type MAO-B. No major changes were observed in the k(cat) values of these chimeras. From the data obtained, two sequences, i.e. 62-103 and 146-220, appeared of particular importance in constituting the binding site of MAO-B. On the other hand, the catalytic properties and specificity of MAO-A appeared to be relatively insensitive to substitution of both the NH2- (up to position 112) and COOH-termini (from residue 395) of MAO-A with the corresponding MAO-B sequences. However, further modification of the central 283-residue sequence of MAO-A did not appear compatible with enzymic activity. None of the engineered chimeras showed a shift in specificity from one isoform to the other.
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页码：934 / 942
页数：9
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