DNA UNWINDING ACTIVITY OF REPLICATION PROTEIN-A

被引：89

作者：

GEORGAKI, A ^{[1
]}

STRACK, B ^{[1
]}

PODUST, V ^{[1
]}

HUBSCHER, U ^{[1
]}

机构：

[1] UNIV ZURICH IRCHEL, DEPT PHARMACOL & BIOCHEM, WINTERTHURERSTR 190, CH-8057 ZURICH, SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 308卷 / 03期

关键词：

REPLICATION PROTEIN-A; DNA UNWINDING; INITIATION; DNA REPLICATION;

D O I：

10.1016/0014-5793(92)81283-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replication protein A (RP-A) is a heterotrimeric complex conserved in eukaryotic cells. It binds to single-stranded DNA and is essential for initiation and elongation of DNA replication. In this communication we give evidence that this protein can unwind DNA independent of magnesium and ATP, two essential cofactors for bona fide DNA helicase activity. RP-A can unwind up to at least 350 basepairs and appears to be required in stoichiometric amounts. The reaction is extremely sensitive to NaCl and MgCl2. This activity of RF-A is suggestive for a possible unwinding function in initiation of DNA replication in eukaryotes.

引用

页码：240 / 244

页数：5

共 19 条

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