A RAPID METHOD FOR MONITORING DNA LABELING REACTIONS WITH HAPTENS

被引：2

作者：

CORTI, A

TRIPPUTI, P

CASSANI, G

机构：

[1] Tecnogen SpA, 20131 Milan

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 134卷 / 01期

关键词：

Enzyme immunoassay; Hybridisation assay; Nonradioactive labelling; Nucleic acid;

D O I：

10.1016/0022-1759(90)90114-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes. © 1990.

引用

页码：81 / 86

页数：6

共 6 条

[1] RAPID AND SENSITIVE COLORIMETRIC METHOD FOR VISUALIZING BIOTIN-LABELED DNA PROBES HYBRIDIZED TO DNA OR RNA IMMOBILIZED ON NITROCELLULOSE - BIO-BLOTS [J].