RAPID ISOLATION OF ANY KNOWN GENES FROM WHOLE CELLS OF YEAST BY PCR

被引：6

作者：

RAMOTAR, D

机构：

[1] Environment and Health, CHUL Research Centre, Ste-Foy, G1V 4G2, Quebec, 2705 Boul, Laurier

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1995年 / 145卷 / 02期

关键词：

YEAST; POLYMERASE CHAIN REACTION; GENE; METHOD;

D O I：

10.1007/BF00935491

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

引用

页码：185 / 187

页数：3