PCR JUMPING IN CLONES OF 30-MILLION-YEAR-OLD DNA FRAGMENTS FROM AMBER PRESERVED TERMITES (MASTOTERMES-ELECTRODOMINICUS)

被引：59

作者：

DESALLE, R ^{[1
]}

BARCIA, M ^{[1
]}

WRAY, C ^{[1
]}

机构：

[1] YALE UNIV,DEPT GEOL & GEOPHYS,NEW HAVEN,CT 06511

来源：

EXPERIENTIA | 1993年 / 49卷 / 10期

关键词：

POLYMERASE CHAIN REACTION; ANCIENT DNA; TERMITE RELATIONSHIPS; MASTOTERMES-ELECTRODOMINICUS;

D O I：

10.1007/BF01952607

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA from 30-million-year-old amber preserved termites (Mastotermes electrodominicus) was PCR amplified with nuclear ribosomal RNA small subunit primers and cloned into the TA vector (INVITROGEN). We obtained several classes of recombinant clones as a result. Authentic Mastotermes electrodominicus clones were identified. The source of other classes of clones was identified as contaminants of the ancient DNA template. Several of the clones appeared to be chimeric in structure with half of the clone identical to the termite sequence and the other half identical to contaminant sequences. The phenomenon of PCR jumping was identified as a possible source for the chimeric clones.

引用

页码：906 / 909

页数：4

共 22 条

[1]

BRADLEY RD, 1992, COMMUNICATION

[2]

CANO R J, 1992, Medical Science Research, V20, P619

[3]

CANO R J, 1992, Medical Science Research, V20, P249

[4] AMPLIFICATION AND SEQUENCING OF DNA FROM A 120-135-MILLION-YEAR-OLD WEEVIL [J].