QUANTITATIVE ESTIMATION OF NEUTRAL SUGARS BY GAS-LIQUID CHROMATOGRAPHY

This paper reports an improved method for separating by isothermal gas-liquid chromatography, the hexoses, the pentoses, five deoxy sugars associated with natural products, and erythrose, as their alditol acetates. The solvent peak shows virtually no tailing, thus making quantitative estimation of the pentose sugars as accurate as that of the hexoses. It is also shown that the surface characteristics of the solid support to a large extent determine the degree of resolution of the various sugars. The conditions described give sharp, well resolved, and relatively narrow peaks for the slower moving components, thus improving the accuracy with which they can be measured quantitatively. © 1969.