USE OF LIVING COLUMNS TO SELECT SPECIFIC PHAGE ANTIBODIES

被引：40

作者：

BRADBURY, A

PERSIC, L

WERGE, T

CATTANEO, A

机构：

[1] CNR, IST NEUROBIOL, I-00156 ROME, ITALY

[2] SOCIETA ITALIANA RICERCA SCI, I-34012 TRIESTE, ITALY

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 13期

关键词：

D O I：

10.1038/nbt1293-1565

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we demonstrate that it is possible to confront two recombinant microorganisms in order to select one using the other. We have shown that an epitope derived from p21ras expressed within the outer membrane protein, LamB, can be recognized both by the monoclonal antibody Y13-259, as well as the single chain Fv fragment derived from it. This specificity, which is maintained when the Y13-259 single chain Fv is expressed as a fusion protein with the phage fd gene 3 protein, has allowed us to use the living column of LamB-ras to purify Y13-259 phage from a background of non-binding phage, even at dilutions as high as 10 phage in 10(10) irrelevant phage.

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页码：1565 / 1569

页数：5

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