EVIDENCE THAT THE PROTEIN-COMPONENTS OF BOVINE ERYTHROCYTE GREEN HEME BINDING-PROTEIN AND FLAVIN REDUCTASE ARE IDENTICAL

被引:16
作者
QUANDT, KS [1 ]
XU, F [1 ]
CHEN, P [1 ]
HULTQUIST, DE [1 ]
机构
[1] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48109
关键词
D O I
10.1016/0006-291X(91)91816-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bovine erythrocyte green heme binding protein and bovine erythrocyte flavin reductase have been isolated in highly purified forms and subjected to amino acid analysis and N-terminal amino acid sequence analysis. The two proteins possess similar amino acid compositions and identical N-terminal amino acid sequences. Moreover, the two proteins are immunochemically cross-reactive and are indistinguishable when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by double diffusion technique. This study provides evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical. © 1991.
引用
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页码:315 / 321
页数:7
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