APPLICATION OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO THE SEPARATION OF APOLIPOPROTEIN-A-IV, APOLIPOPROTEIN-A-I AND APOLIPOPROTEIN-E FROM RAT HIGH-DENSITY-LIPOPROTEIN

被引：2

作者：

MEYER, B ^{[1
]}

KECORIUS, E ^{[1
]}

BARTER, P ^{[1
]}

FIDGE, N ^{[1
]}

TETAZ, T ^{[1
]}

机构：

[1] BAKER MED RES INST,POB 348,PRAHRAN,VIC 3181,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 540卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88827-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Apolipoproteins A-IV, A-I and E from rat high-density lipoprotein (HDL) were successfully purified by reversed-phase high-performance liquid chromatography (RP-HPLC), using a method which we have previously developed for the separation of apolipoproteins A-IV, A-I and E from human lymph chylomicrons [T. Tetaz, E. Kecorius, B. Grego and N. Fidge, J. Chromatogr., 511 (1990) 147]. Since analyticalscale RP-HPLC indicated that the C apolipoproteins from rat HDL coeluted with both apo A-IV and apo A-I, delipidated rat HDL was first subjected to preparative-scale size-exclusion HPLC (HPSEC) on a Serva Si300 column, which effectively separated the C apolipoproteins from all but apolipoprotein E. Fractions from HPSEC which were enriched for apolipoproteins A-IV, A-I or E were directly applied to RP-HPLC on a TSK Phenyl-5PW column. This procedure yielded fractions containing apolipoproteins A-IV, A-I or E which were pure as assessed by N-terminal sequencing and silver staining of sodium dodecyl sulphate-polyacrylamide gels.

引用

页码：386 / 391

页数：6

共 6 条

[1] IDENTIFICATION OF PROAPOA-I IN RAT LYMPH AND PLASMA - METABOLIC CONVERSION TO MATURE APOA-I [J].