TRANSPLANTATION OF STORED ALLOGENEIC BONE MARROW IN DOGS SELECTED BY HISTOCOMPATIBILITY TYPING

Allogeneic canine marrow preserved at —80 C in 10% dimethyl sulfoxide was used for transplantation in unrelated dogs following 1200 R whole body irradiation. Lymphocytes of donors and recipients were typed with four canine cytotoxic isoantisera. The results in 10 recipients that were matched with their donors were compared to the results in 10 mismatched recipients. A successful marrow graft was observed in all 10 matched recipients and in 9 of 10 mismatched recipients. Graft rejection occurred in 2 of 10 matched and 7 of 10 mismatched recipients. The matched recipients survived significantly longer than did the mismatched ones (P < 0.005). Two of the matched dogs are alive more than 135 and 160 days after transplantation. The cryopreservation of the marrow did not appear to lessen its ability to produce lethal graft-versus-host disease. The results d emonstrate the importance of histocompatibility matching in successful grafting with cryopreserved marrow. © 1969 by the Williams and Wilkins Co.