STUDIES ON ANTISENSE INHIBITION OF TRANSLATION INVITRO - ANOMALIES AND REEVALUATION

被引：7

作者：

RICKER, RD ^{[1
]}

KAJI, A ^{[1
]}

机构：

[1] UNIV PENN, SCH MED, DEPT MICROBIOL, 258 JOHNSON PAVILL, PHILADELPHIA, PA 19104 USA

来源：

FEBS LETTERS | 1992年 / 309卷 / 03期

关键词：

ANTISENSE; TRANSLATIONAL REGULATION; DNA OLIGOMER; RNA OLIGOMER;

D O I：

10.1016/0014-5793(92)80808-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Experiments were carried out to better characterize antisense control of translation. Results in an E. coli system confirmed specific inhibition of poly(U) translation. At low concentrations, certain homopolymers (including poly(rA)) stimulated translation. Oligo(dA(n)) was inhibitory at n less-than-or-equal-to 8. Translation of globin mRNA in reticulocyte lysates indicated that ssDNA 15-mers targeted at beta-globin mRNA inhibited both alpha- and beta-globin production. Sequences targeted immediately downstream of the AUG were the least effective in inhibition. These and other anomalies are discussed here in relation to those of others, emphasizing caution in performing antisense experiments.

引用

页码：363 / 370

页数：8

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