USE OF SELF-SUSTAINED SEQUENCE REPLICATION AMPLIFICATION REACTION TO ANALYZE AND DETECT MUTATIONS IN ZIDOVUDINE-RESISTANT HUMAN-IMMUNODEFICIENCY-VIRUS

被引:55
作者
GINGERAS, TR
PRODANOVICH, P
LATIMER, T
GUATELLI, JC
RICHMAN, DD
BARRINGER, KJ
机构
[1] UNIV CALIF SAN DIEGO,BAXTER DIAGNOST INC,DEPT MED,SAN DIEGO,CA 92103
[2] VET ADM MED CTR,SAN DIEGO,CA 92161
关键词
D O I
10.1093/infdis/164.6.1066
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mutations at amino acid positions 67, 70, 215, and 219 in the human immunodeficiency virus type 1 (HIV-1) pol gene correlate with the emergence of resistance to zidovudine (AZT). These four positions were monitored in viral RNA extracted from infected peripheral blood mononuclear cells (PBMC) and viral stocks obtained after coculture with uninfected lymphocytes. Genotype determinations were made using the self-sustained sequence replication (3SR) and differential bead-based sandwich hybridization (BBSH) assay. The hybridization results obtained by 3SR and BBSH analyses were verified by dideoxynucleotide sequencing of the 3SR products. Correlation of 3SR and BBSH with polymerase chain reaction and Southern hybridization analyses of the PBMC and corresponding viral isolates indicated that PBMC and corresponding HIV-1 isolates may differ in their genotypes at the monitored amino acid positions, variations from the wild-type nucleotide sequence may occur proximal to the codons being monitored, and viral isolates possessing the same genotypes at the four monitored amino acid positions showed a threefold variation in their ID50 measurements.
引用
收藏
页码:1066 / 1074
页数:9
相关论文
共 18 条
[1]  
BOUCHER CA, 1990, LANCET, P585
[2]   DETECTION OF SICKLE-CELL BETA-S-GLOBIN ALLELE BY HYBRIDIZATION WITH SYNTHETIC OLIGONUCLEOTIDES [J].
CONNER, BJ ;
REYES, AA ;
MORIN, C ;
ITAKURA, K ;
TEPLITZ, RL ;
WALLACE, RB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :278-282
[3]   DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN AIDS PATIENTS USING AMPLIFICATION-MEDIATED HYBRIDIZATION ANALYSES - REPRODUCIBILITY AND QUANTITATIVE LIMITATIONS [J].
DAVIS, GR ;
BLUMEYER, K ;
DIMICHELE, LJ ;
WHITFIELD, KM ;
CHAPPELLE, H ;
RIGGS, N ;
GHOSH, SS ;
KAO, PM ;
FAHY, E ;
KWOH, DY ;
GUATELLI, JC ;
SPECTOR, SA ;
RICHMAN, DD ;
GINGERAS, TR .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (01) :13-20
[4]   THE HUMAN IMMUNODEFICIENCY VIRUS - INFECTIVITY AND MECHANISMS OF PATHOGENESIS [J].
FAUCI, AS .
SCIENCE, 1988, 239 (4840) :617-622
[5]   DETECTION OF SINGLE DNA-BASE DIFFERENCES BY COMPETITIVE OLIGONUCLEOTIDE PRIMING [J].
GIBBS, RA ;
NGUYEN, PN ;
CASKEY, CT .
NUCLEIC ACIDS RESEARCH, 1989, 17 (07) :2437-2448
[6]  
GINGERAS TR, 1990, ANN BIOL CLIN-PARIS, V48, P498
[7]   ISOTHERMAL, INVITRO AMPLIFICATION OF NUCLEIC-ACIDS BY A MULTIENZYME REACTION MODELED AFTER RETROVIRAL REPLICATION [J].
GUATELLI, JC ;
WHITFIELD, KM ;
KWOH, DY ;
BARRINGER, KJ ;
RICHMAN, DD ;
GINGERAS, TR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1874-1878
[8]   HIV WITH REDUCED SENSITIVITY TO ZIDOVUDINE (AZT) ISOLATED DURING PROLONGED THERAPY [J].
LARDER, BA ;
DARBY, G ;
RICHMAN, DD .
SCIENCE, 1989, 243 (4899) :1731-1734
[9]   MULTIPLE MUTATIONS IN HIV-1 REVERSE-TRANSCRIPTASE CONFER HIGH-LEVEL RESISTANCE TO ZIDOVUDINE (AZT) [J].
LARDER, BA ;
KEMP, SD .
SCIENCE, 1989, 246 (4934) :1155-1158
[10]  
Maniatis T., 1982, MOL CLONING