DETECTION OF GEMINIVIRUSES FROM TROPICAL COUNTRIES BY A DOUBLE MONOCLONAL-ANTIBODY ELISA USING ANTIBODIES TO AFRICAN CASSAVA MOSAIC-VIRUS

被引：11

作者：

GIVORD, L

FARGETTE, D

KOUNOUNGUISSA, B

THOUVENEL, JC

WALTER, B

VANREGENMORTEL, MHV

机构：

[1] Immunochimie, Institut de Biologie Moléculaire et Cellulaire, CNRS (IBMC), F67084 Strasbourg cedex, 15, rue René-Descartes

[2] LPRC-ORSTOM, CIRAD, F34032 Montpellier cedex

[3] INRA, F68021 Colmar

[4] ORSTOM, Dokki Cairo, midan Ibn Afan

来源：

AGRONOMIE | 1994年 / 14卷 / 05期

关键词：

GEMINIVIRUS-III; MONOCLONAL ANTIBODY; ELISA; AFRICAN CASSAVA MOSAIC VIRUS; TOMATO YELLOW LEAF CURL VIRUS;

D O I：

10.1051/agro:19940506

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, monoclonal antibodies (mAbs), which were prepared against particles of a Nigerian isolate of African cassava mosaic virus (ACMV), differentiated African geminiviruses (tomato yellow leaf curl virus, TYLCV; ACMV from West and East Africa; and okra leaf curl virus) from those originating in other countries of the world (euphorbia mosaic virus; Indian cassava mosaic virus; and TYLCV (Indian isolate)). All these viruses belong to the whitefly-transmitted geminivirus subgroup III. One mAb reacted with all 7 tested viruses and isolates. For detecting TYLCV from Senegal, it proved necessary to use a reducing agent in extraction buffer. A diagnostic DAS-ELISA was developed. This relies only on the use of mAbs and is useful for large-scale field screening.

引用

页码：327 / 333

页数：7