A CYTOSOLIC SPERM FACTOR TRIGGERS CALCIUM OSCILLATIONS AND MEMBRANE HYPERPOLARIZATIONS IN HUMAN OOCYTES

被引:154
作者
HOMA, ST
SWANN, K
机构
[1] ST BARTHOLOMEWS HOSP, DEPT OBSTET & GYNAECOL, IVF UNIT, LONDON EC1A 7BE, ENGLAND
[2] UCL, DEPT ANAT & DEV BIOL, LONDON WC1E 6BT, ENGLAND
基金
英国医学研究理事会;
关键词
CALCIUM; FERTILIZATION; MEMBRANE POTENTIAL; OOCYTE; SPERMATOZOA;
D O I
10.1093/oxfordjournals.humrep.a138452
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Intracellular free Ca2+ concentrations ([Ca2+](i)) and membrane potentials were measured in mature human oocytes. Injection of cytosolic extracts made from human or hamster spermatozoa triggered oscillations in [Ca2+](i) in human oocytes similar to those described previously in mouse and hamster oocytes. In contrast, injection of carrier buffer caused no [Ca2+](i) increase and injection of Ca2+-containing solutions caused only a single [Ca2+](i) transient. Injection of human sperm extracts also triggered [Ca2+](i) oscillations in mature mouse oocytes. The [Ca2+](i) oscillations in human oocytes were accompanied by hyperpolarizations in membrane potential. Perfusing oocytes with the sulphydryl reagent thimerosal also caused oscillations in the free [Ca2+](i) concentration simultaneously with membrane potential hyperpolarizations. These data suggest that human oocytes possess a similar mechanism for generating [Ca2+](i) oscillations to those described in other mammalian oocytes and a membrane potential response similar to that seen previously specifically in hamster oocytes. The data also support the view that human oocytes are activated at fertilization by diffusion of a protein from the spermatozoa into the ooplasm after gamete membrane fusion.
引用
收藏
页码:2356 / 2361
页数:6
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