DIRECT OBSERVATION OF TOXIC EFFECTS OF CYANOBACTERIAL EXTRACELLULAR PRODUCTS ON DAPHNIA

The filtrate from a suspension of the cyanobacterium Anabaena minutissima var. attenuata depressed thoracic limb beat frequency of Daphnia carinata by 38-45% in a food-free medium. Repeated exposure to the filtrate produced a similar depression of activity with full or nearly full recovery. Response time to the filtrate was 5-8 min and recovery time was 8-12 min. The dose effect on limb beat frequency was continuous, linear, correlated with increased concentration and with no threshold. There was no relationship between body length and limb beat frequency. The interaction between toxicity and food concentration was tested using the diatom Cyclotella suspended in Anabaena filtrate. Daphnia limb beat frequency was depressed by 62%.