NACL-DEPENDENT EXPRESSION OF AMILORIDE-BLOCKABLE NA+ CHANNEL IN XENOPUS OOCYTES

被引:24
作者
ASHER, C
SINGER, D
EREN, R
YEGER, O
DASCAL, N
GARTY, H
机构
[1] WEIZMANN INST SCI, DEPT MEMBRANE RES & BIOPHYS, IL-76100 REHOVOT, ISRAEL
[2] TEL AVIV UNIV, SACKLER SCH MED, DEPT PHYSIOL & PHARMACOL, TEL AVIV, ISRAEL
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 02期
关键词
ALDOSTERONE; CHICKEN INTESTINE; COLON; COPRODEUM; EPITHELIA; SODIUM DIET; RIBONUCLEIC ACID; SODIUM TRANSPORT;
D O I
10.1152/ajpgi.1992.262.2.G244
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
RNA was isolated from chicken lower intestine (both colon and coprodeum) and injected into Xenopus oocytes. Na-22+ fluxed measured after 1-4 days demonstrated the induction of an amiloride-blockable pathway. The Na+ transporter expressed by the exogenous RNA had a high affinity to amiloride (inhibitory constant < 0.1-mu-M), but was insensitive to ethylisopropyl amiloride, i.e., it is likely to be the apical Na+ channel. Functional channels were readily expressed in oocytes injected with RNA derived from chickens fed a low-NaCl diet. On the other hand, no channel activity was detected in oocytes injected with RNA isolated from chickens fed a high-NaCl diet. Thus the previously reported regulation of transport by the dietary NaCl intake involves modulations in the level of mRNA that codes either for the Na+ channel or a posttranscriptional regulator of the channel.
引用
收藏
页码:G244 / G298
页数:55
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